# TOP STRAND, S1 # Oligo ID: R778 # Sequence (5'to 3'): GCAGTGGATGTGAGA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R781 # Sequence (5'to 3'): TCTCACATCCACTGC # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: P4 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.0005 # [KCl] = 0.050 # [Tris] = 0.010 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 02-10-2003 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- .1220000000E+02 .1271274575E-01 .5764005114E-02 .4412458187E-02 .1230000000E+02 .1160721962E-01 .3100878609E-02 .2309505281E-02 .1240000000E+02 .1281561950E-01 .4425823532E-02 .3640925625E-02 .1250000000E+02 .1151212150E-01 .3612451377E-02 .3205383500E-02 .1260000000E+02 .1265013800E-01 .5318160327E-02 .4361356500E-02 .1270000000E+02 .1143226162E-01 .5514841203E-02 .4450895469E-02 .1280000000E+02 .1077031250E-01 .3705766535E-02 .3245527500E-02 .1290000000E+02 .1243090538E-01 .3230723281E-02 .2740809625E-02 .1300000000E+02 .1285322688E-01 .4167584976E-02 .3008872344E-02 .1310000000E+02 .1059412388E-01 .4652684504E-02 .3530885156E-02 .1320000000E+02 .8934233875E-02 .4917115047E-02 .3790777906E-02 .1330000000E+02 .1050363438E-01 .4048051171E-02 .3018593125E-02 .1340000000E+02 .8903801375E-02 .2662897293E-02 .2117887531E-02 .1350000000E+02 .1035064513E-01 .2254533188E-02 .1801823594E-02 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