# TOP STRAND, S1 # Oligo ID: R778 # Sequence (5'to 3'): GCAGTGGATGTGAGA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R781 # Sequence (5'to 3'): TCTCACATCCACTGC # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: P5 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.0015 # [KCl] = 0.050 # [Tris] = 0.010 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 09-19-2002 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- .1760000000E+02 .1772818025E-01 .1149686901E-01 .8180622187E-02 .1770000000E+02 .1628146600E-01 .9434692536E-02 .7134460500E-02 .1780000000E+02 .1655531062E-01 .1034057541E-01 .7642892031E-02 .1790000000E+02 .1642136312E-01 .9167294995E-02 .6770951875E-02 .1800000000E+02 .1638231638E-01 .8575339601E-02 .6103008625E-02 .1810000000E+02 .1576875012E-01 .8699876153E-02 .6174552375E-02 .1820000000E+02 .1528088000E-01 .8914836665E-02 .6690675000E-02 .1830000000E+02 .1539530688E-01 .9174241615E-02 .6586992344E-02 .1840000000E+02 .1443095550E-01 .9657024084E-02 .7015032000E-02 .1850000000E+02 .1410251525E-01 .9589105650E-02 .6994343563E-02 .1860000000E+02 .1428010137E-01 .1064857141E-01 .7525648969E-02 .1870000000E+02 .1399358262E-01 .1036968909E-01 .7465363031E-02 .1880000000E+02 .1372643937E-01 .9204987295E-02 .6299775625E-02 .1890000000E+02 .1497571250E-01 .8808862469E-02 .5887198125E-02 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