# TOP STRAND, S1 # Oligo ID: R778 # Sequence (5'to 3'): GCAGTGGATGTGAGA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R781 # Sequence (5'to 3'): TCTCACATCCACTGC # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: P6 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.003 # [KCl] = 0.050 # [Tris] = 0.010 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 03-07-2003 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- .1570000000E+02 .1359373200E-01 .9771330564E-02 .6959032500E-02 .1580000000E+02 .1248147850E-01 .9564675472E-02 .7135839375E-02 .1590000000E+02 .1065881988E-01 .7760219285E-02 .5920930156E-02 .1600000000E+02 .1380649163E-01 .9585429051E-02 .7177263375E-02 .1610000000E+02 .1309886764E-01 .8935045931E-02 .7078627953E-02 .1620000000E+02 .1276491688E-01 .8090214821E-02 .6281228906E-02 .1630000000E+02 .1245175075E-01 .9010970713E-02 .6552844063E-02 .1640000000E+02 .1158176674E-01 .8660277198E-02 .6615782394E-02 .1650000000E+02 .1036108050E-01 .9230264401E-02 .7144974375E-02 .1660000000E+02 .8330165375E-02 .7670146567E-02 .5645898187E-02 .1670000000E+02 .1051669487E-01 .8163506417E-02 .5961716406E-02 .1680000000E+02 .1002066950E-01 .8355184579E-02 .6859620625E-02 .1690000000E+02 .1065940857E-01 .9735881346E-02 .7293029286E-02 .1700000000E+02 .9418358762E-02 .8059381161E-02 .6136839047E-02 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