# TOP STRAND, S1 # Oligo ID: R778 # Sequence (5'to 3'): GCAGTGGATGTGAGA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R781 # Sequence (5'to 3'): TCTCACATCCACTGC # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: PN # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.000 # [KCl] = 0.050 # [Tris] = 0.010 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 12-06-2001 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- .1240000000E+02 .2702286375E-02 .2599222558E-02 .1920805031E-02 .1250000000E+02 .3039539150E-02 .2537048000E-02 .2065878600E-02 .1260000000E+02 .1386014137E-02 .1718785358E-02 .1356266363E-02 .1270000000E+02 .1926629412E-02 .3452584387E-02 .2663137044E-02 .1280000000E+02 .1040803219E-02 .1697517557E-02 .1315070336E-02 .1290000000E+02 .1776215088E-02 .1882997179E-02 .1553770684E-02 .1300000000E+02 .1289523750E-02 .3356417731E-02 .2826797750E-02 .1310000000E+02 .2302240963E-02 .2661032094E-02 .2012979278E-02 .1320000000E+02 .4379517875E-03 .3006446737E-02 .2174890634E-02 .1330000000E+02 .8547100125E-03 .1958940010E-02 .1530562491E-02 .1340000000E+02 .1699083484E-02 .2694171556E-02 .2052596137E-02 .1350000000E+02 .3723598037E-03 .2284049360E-02 .1845486147E-02 .1360000000E+02 .6377572750E-03 .1836677946E-02 .1162676619E-02 .1370000000E+02 .1308873675E-02 .3275049035E-02 .2088346325E-02 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