# TOP STRAND, S1 # Oligo ID: R778 # Sequence (5'to 3'): GCAGTGGATGTGAGA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R781 # Sequence (5'to 3'): TCTCACATCCACTGC # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: Q6 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.0015 # [KCl] = 0.600 # [Tris] = 0.010 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 09-05-2003 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- .1820000000E+02 .5539534012E-02 .6115069359E-02 .4791311984E-02 .1830000000E+02 .6315385125E-02 .4447544908E-02 .2913822094E-02 .1840000000E+02 .6596829000E-02 .5458100071E-02 .4101926500E-02 .1850000000E+02 .5661023000E-02 .4971910373E-02 .3115228500E-02 .1860000000E+02 .5434416250E-02 .4620415507E-02 .2817218313E-02 .1870000000E+02 .5472170250E-02 .4120231943E-02 .2971775937E-02 .1880000000E+02 .5857335000E-02 .4713311235E-02 .3216187250E-02 .1890000000E+02 .5945889887E-02 .4313411727E-02 .3039466613E-02 .1900000000E+02 .3629644875E-02 .4902916007E-02 .3324744156E-02 .1910000000E+02 .6092437625E-02 .4514780314E-02 .3210374719E-02 .1920000000E+02 .3485983838E-02 .3966417249E-02 .2897114703E-02 .1930000000E+02 .3799754875E-02 .4586916358E-02 .3271237875E-02 .1940000000E+02 .4367102500E-02 .3282518440E-02 .2112935125E-02 .1950000000E+02 .6121940000E-02 .2367515282E-02 .1787232250E-02 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