# TOP STRAND, S1 # Oligo ID: R778 # Sequence (5'to 3'): GCAGTGGATGTGAGA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R781 # Sequence (5'to 3'): TCTCACATCCACTGC # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: R4 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.010 # [KCl] = 0.100 # [Tris] = 0.010 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 08-18-2003 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- .1930000000E+02 -.6131985750E-02 .3396658668E-01 .2734443325E-01 .1940000000E+02 -.5472309500E-02 .3056219133E-01 .2407505537E-01 .1950000000E+02 -.4177334250E-02 .3353589375E-01 .2655276831E-01 .1960000000E+02 -.2409095625E-02 .3450395716E-01 .2717579503E-01 .1970000000E+02 -.1533509337E-02 .3329019141E-01 .2673275500E-01 .1980000000E+02 -.2583990250E-02 .3342751221E-01 .2668921231E-01 .1990000000E+02 -.1946792250E-02 .3163451137E-01 .2549536725E-01 .2000000000E+02 -.4249802750E-02 .3018185111E-01 .2464691525E-01 .2010000000E+02 -.1637852250E-02 .3140066016E-01 .2601937225E-01 .2020000000E+02 -.1975179750E-02 .2987063734E-01 .2505670975E-01 .2030000000E+02 -.7305500000E-04 .2861379933E-01 .2286300650E-01 .2040000000E+02 -.3431756250E-03 .2793900788E-01 .2230563728E-01 .2050000000E+02 -.2166014039E-02 .2740269562E-01 .2153010247E-01 .2060000000E+02 .2950110000E-03 .2799035532E-01 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