# TOP STRAND, S1 # Oligo ID: R778 # Sequence (5'to 3'): GCAGTGGATGTGAGA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R781 # Sequence (5'to 3'): TCTCACATCCACTGC # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: R6 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.010 # [KCl] = 0.600 # [Tris] = 0.010 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 08-27-2003 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- .1820000000E+02 .6421192450E-02 .1899812670E-01 .1506552255E-01 .1830000000E+02 .6206791625E-02 .1740844563E-01 .1384718338E-01 .1840000000E+02 .5374291000E-02 .1756980061E-01 .1398674975E-01 .1850000000E+02 .5522224737E-02 .1759391148E-01 .1383537026E-01 .1860000000E+02 .5946399700E-02 .1767308012E-01 .1359981938E-01 .1870000000E+02 .5396718125E-02 .1624993996E-01 .1289820934E-01 .1880000000E+02 .6233164125E-02 .1724033492E-01 .1403600838E-01 .1890000000E+02 .6263357875E-02 .1657707258E-01 .1322350441E-01 .1900000000E+02 .5557206713E-02 .1633656065E-01 .1293635286E-01 .1910000000E+02 .5314725560E-02 .1652230675E-01 .1304925830E-01 .1920000000E+02 .6681401250E-02 .1569046803E-01 .1171550625E-01 .1930000000E+02 .7537878875E-02 .1581798308E-01 .1200935941E-01 .1940000000E+02 .6201919162E-02 .1649466792E-01 .1332764084E-01 .1950000000E+02 .6167737250E-02 .1539709949E-01 .1213778994E-01 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