# TOP STRAND, S1 # Oligo ID: R778 # Sequence (5'to 3'): GCAGTGGATGTGAGA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R781 # Sequence (5'to 3'): TCTCACATCCACTGC # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: R7 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.010 # [KCl] = 1.000 # [Tris] = 0.010 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 09-02-2003 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- .1990000000E+02 .1485735250E-01 .1060215293E-01 .8679357500E-02 .2000000000E+02 .1393379275E-01 .9194914637E-02 .7949687250E-02 .2010000000E+02 .1356159200E-01 .8523020783E-02 .7072733000E-02 .2020000000E+02 .1332564250E-01 .9022452326E-02 .7412277500E-02 .2030000000E+02 .1409476475E-01 .7127331964E-02 .5460585250E-02 .2040000000E+02 .1359633675E-01 .7135422696E-02 .5501478250E-02 .2050000000E+02 .1217345750E-01 .6352733984E-02 .5360897500E-02 .2060000000E+02 .1066406675E-01 .7972446839E-02 .6726373250E-02 .2070000000E+02 .1133682900E-01 .8773659647E-02 .7078916000E-02 .2080000000E+02 .1040138500E-01 .7564678443E-02 .6224890000E-02 .2090000000E+02 .1133756450E-01 .6866706258E-02 .5594415500E-02 .2100000000E+02 .1008038550E-01 .6431173706E-02 .5362884500E-02 .2110000000E+02 .1061433250E-01 .6553601150E-02 .4802872500E-02 .2120000000E+02 .1039282550E-01 .6757050656E-02 .5045829500E-02 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