# TOP STRAND, S1 # Oligo ID: R778 # Sequence (5'to 3'): GCAGTGGATGTGAGA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R781 # Sequence (5'to 3'): TCTCACATCCACTGC # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: W4 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.000 # [KCl] = 0.100 # [Tris] = 0.010 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 10-10-2003 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- .1610000000E+02 .1311422925E-01 .8485263526E-02 .7572123250E-02 .1620000000E+02 .1255867488E-01 .1039664005E-01 .8839455125E-02 .1630000000E+02 .1242644000E-01 .8618828464E-02 .7061885000E-02 .1640000000E+02 .1159840350E-01 .9802665864E-02 .8081951500E-02 .1650000000E+02 .1352902500E-01 .6886494152E-02 .5621455000E-02 .1660000000E+02 .1333287838E-01 .9754688111E-02 .8037794125E-02 .1670000000E+02 .1217981400E-01 .9552244934E-02 .7668143500E-02 .1680000000E+02 .8946680162E-02 .9755968307E-02 .8226187337E-02 .1690000000E+02 .1186323416E-01 .8294336506E-02 .6067970419E-02 .1700000000E+02 .1145582900E-01 .9920766087E-02 .8227216000E-02 .1710000000E+02 .1138940300E-01 .9617602215E-02 .8004944500E-02 .1720000000E+02 .1167315400E-01 .6538351804E-02 .5347531000E-02 .1730000000E+02 .1135914313E-01 .7879706628E-02 .6124104375E-02 .1740000000E+02 .1293557387E-01 .7733449527E-02 .6833391125E-02 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