# TOP STRAND, S1 # Oligo ID: R778 # Sequence (5'to 3'): GCAGTGGATGTGAGA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R781 # Sequence (5'to 3'): TCTCACATCCACTGC # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: W7 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.000 # [KCl] = 1.000 # [Tris] = 0.010 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 09-23-2003 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- .1800000000E+02 .2933303500E-02 .1350276837E-01 .8831426750E-02 .1810000000E+02 .1564738500E-02 .1211573509E-01 .7720714250E-02 .1820000000E+02 .3859613750E-02 .1204615795E-01 .8202541875E-02 .1830000000E+02 .1746643450E-02 .1213734451E-01 .8008530550E-02 .1840000000E+02 .3468378000E-02 .1184637058E-01 .7692694000E-02 .1850000000E+02 .3132827750E-02 .9368143520E-02 .6606738875E-02 .1860000000E+02 .2927133000E-02 .9433135956E-02 .6474996500E-02 .1870000000E+02 .1459335750E-02 .8329128513E-02 .5691616875E-02 .1880000000E+02 .2181063000E-02 .8713706259E-02 .5804922500E-02 .1890000000E+02 .3331988750E-02 .7885968653E-02 .5645585250E-02 .1900000000E+02 .3073926500E-02 .7148182914E-02 .5266161250E-02 .1910000000E+02 .4052065250E-02 .7993057385E-02 .5700158625E-02 .1920000000E+02 .3179696000E-02 .6602445701E-02 .4315294000E-02 .1930000000E+02 .4794264750E-02 .5689749142E-02 .3834550875E-02 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