# TOP STRAND, S1 # Oligo ID: R791 # Sequence (5'to 3'): GACCTGACGTGGACCGCTCCTGGGCGTGGT # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R794 # Sequence (5'to 3'): ACCACGCCCAGGAGCGGTCCACGTCAGGTC # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: Z9 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.020 # [KCl] = 0.0 # [Tris] = 0.002 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 05-03-2004 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- .2780000000E+02 .2844213000E-01 .1089056725E-01 .7416905000E-02 .2790000000E+02 .2925378000E-01 .8479338104E-02 .6122230000E-02 .2800000000E+02 .2951028250E-01 .1034723537E-01 .7669353750E-02 .2810000000E+02 .2717255500E-01 .5506266673E-02 .4069977500E-02 .2820000000E+02 .2645936500E-01 .1006296904E-01 .7284492500E-02 .2830000000E+02 .2536270500E-01 .5892033955E-02 .4230302500E-02 .2840000000E+02 .2816750750E-01 .5436607601E-02 .4043241250E-02 .2850000000E+02 .2687380750E-01 .8356579962E-02 .6233966250E-02 .2860000000E+02 .2724467500E-01 .8224780444E-02 .6075892500E-02 .2870000000E+02 .2655726500E-01 .8096355719E-02 .5791807500E-02 .2880000000E+02 .2629370250E-01 .9579557485E-02 .7166418750E-02 .2890000000E+02 .2493136250E-01 .8434867708E-02 .6304978750E-02 .2900000000E+02 .2391388000E-01 .8002671257E-02 .5925360000E-02 .2910000000E+02 .2206922750E-01 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