# TOP STRAND, S1 # Oligo ID: R1441 # Sequence (5'to 3'): TGGCGAGCAC # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R1442 # Sequence (5'to 3'): GTGCTCGCCA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: D1 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.125 # [KCl] = 0.0 # [Tris] = 0.010 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 02-11-2004 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- 1.347135576E+001 -1.185922700E-002 1.312409385E-002 9.428704000E-003 1.356571216E+001 -8.905028000E-003 1.308164834E-002 8.859974667E-003 1.366007292E+001 -7.390968333E-003 1.073542205E-002 8.256422222E-003 1.375443804E+001 -1.085042500E-002 1.496621838E-002 1.088960667E-002 1.384880751E+001 -7.084552333E-003 1.241573251E-002 9.141376889E-003 1.394318134E+001 -7.796118000E-003 1.296354254E-002 9.973896000E-003 1.403755953E+001 -8.959890667E-003 1.063943559E-002 8.163012444E-003 1.413194207E+001 -7.696609767E-003 7.573274998E-003 5.655413644E-003 1.422632897E+001 -5.029361667E-003 6.731314234E-003 4.663056444E-003 1.432072023E+001 -8.143615667E-003 1.007314834E-002 7.166913111E-003 1.441511584E+001 -2.127893367E-003 1.667350259E-002 1.163517776E-002 1.450951581E+001 -2.536340333E-003 1.597460737E-002 1.179416022E-002 1.460392013E+001 -4.845015333E-003 1.662253574E-002 1.122684978E-002 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