# TOP STRAND, S1 # Oligo ID: R1441 # Sequence (5'to 3'): TGGCGAGCAC # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R1442 # Sequence (5'to 3'): GTGCTCGCCA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: Q9 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.0015 # [KCl] = 0.0 # [Tris] = 0.002 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 03-30-2004 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- 1.233941870E+001 -1.916403750E-003 4.331886360E-002 3.472898437E-002 1.243372283E+001 -6.189162500E-003 4.030610183E-002 3.198745875E-002 1.252803131E+001 1.231403750E-003 3.958804646E-002 3.174651812E-002 1.262234416E+001 -2.006850000E-003 3.770081684E-002 2.952221000E-002 1.271666135E+001 -2.392772500E-003 3.332485608E-002 2.593684875E-002 1.281098291E+001 2.830750000E-003 3.988843423E-002 3.215622000E-002 1.290530882E+001 -1.261757000E-003 4.248244859E-002 3.326518600E-002 1.299963908E+001 -9.245135625E-004 3.946044092E-002 3.087000305E-002 1.309397370E+001 -8.128782500E-004 3.779068335E-002 2.978398662E-002 1.318831268E+001 7.803647500E-003 4.091496745E-002 3.252227875E-002 1.328265602E+001 -4.470488750E-004 3.379564905E-002 2.585570444E-002 1.337700371E+001 3.092385500E-003 2.990163751E-002 2.384574225E-002 1.347135576E+001 7.938425000E-005 3.006335659E-002 2.408294787E-002 1.356571216E+001 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