# TOP STRAND, S1 # Oligo ID: R1441 # Sequence (5'to 3'): TGGCGAGCAC # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R1442 # Sequence (5'to 3'): GTGCTCGCCA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: R9 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.010 # [KCl] = 0.0 # [Tris] = 0.002 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 05-04-2004 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- 1.083114512E+001 1.521509812E-002 1.056300287E-002 8.317057344E-003 1.092537955E+001 1.408918909E-002 9.993928314E-003 8.061055913E-003 1.101961833E+001 1.129050811E-002 9.406737966E-003 7.405784859E-003 1.111386147E+001 1.150448050E-002 8.344347995E-003 7.586129500E-003 1.120810896E+001 1.404338262E-002 1.160261990E-002 9.317799875E-003 1.130236081E+001 1.183557775E-002 9.897100763E-003 7.739599750E-003 1.139661702E+001 1.493573300E-002 8.669877467E-003 7.042269500E-003 1.149087759E+001 1.340966613E-002 8.423653959E-003 5.984418875E-003 1.158514251E+001 1.277358625E-002 7.221033364E-003 5.613986250E-003 1.167941178E+001 1.327509025E-002 8.232906095E-003 6.859802250E-003 1.177368542E+001 1.195807850E-002 7.816377565E-003 6.726509000E-003 1.186796341E+001 1.150656738E-002 6.742257365E-003 5.445199469E-003 1.196224575E+001 1.289621316E-002 9.424537150E-003 8.188756838E-003 1.205653246E+001 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