# TOP STRAND, S1 # Oligo ID: R1441 # Sequence (5'to 3'): TGGCGAGCAC # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R1442 # Sequence (5'to 3'): GTGCTCGCCA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: V1 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.050 # [KCl] = 0.0 # [Tris] = 0.010 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 02-22-2004 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- 1.772170676E+001 -9.898447500E-004 9.503676245E-003 6.946467813E-003 1.781625920E+001 -7.452628750E-003 1.183718271E-002 7.915996000E-003 1.791081599E+001 -3.027257500E-004 9.049523111E-003 6.655107875E-003 1.800537715E+001 -6.825022250E-003 6.185999100E-003 4.672981000E-003 1.809994266E+001 -4.278094142E-003 9.140129355E-003 7.164934572E-003 1.819451252E+001 -5.973753600E-003 1.154860256E-002 9.224627300E-003 1.828908675E+001 -5.562957125E-003 1.132990535E-002 8.505572094E-003 1.838366533E+001 -1.270283737E-002 8.294897798E-003 6.594949469E-003 1.847824826E+001 -9.326717875E-003 1.260694274E-002 9.639461594E-003 1.857283555E+001 -6.148092175E-003 5.492258931E-003 4.382613619E-003 1.866742720E+001 -6.822995500E-003 7.396807850E-003 5.789243250E-003 1.876202321E+001 -7.404399875E-003 5.100069544E-003 4.321828656E-003 1.885662357E+001 -5.901561750E-003 7.406644683E-003 5.991140750E-003 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