# TOP STRAND, S1 # Oligo ID: R1441 # Sequence (5'to 3'): TGGCGAGCAC # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R1442 # Sequence (5'to 3'): GTGCTCGCCA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: X9 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.0005 # [KCl] = 0.0 # [Tris] = 0.002 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 03-16-2004 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- 1.403755953E+001 1.243336429E-002 1.163798440E-002 9.198146531E-003 1.413194207E+001 1.169355707E-002 9.711428622E-003 8.313957633E-003 1.422632897E+001 8.700651571E-003 1.109330576E-002 8.721572939E-003 1.432072023E+001 9.127748143E-003 1.000044579E-002 7.548316367E-003 1.441511584E+001 7.242787571E-003 1.010922613E-002 6.871931347E-003 1.450951581E+001 1.088901074E-002 7.855543513E-003 6.566207731E-003 1.460392013E+001 1.021142529E-002 1.093454143E-002 9.337453959E-003 1.469832881E+001 9.661951914E-003 8.889250589E-003 7.386812645E-003 1.479274185E+001 1.291596329E-002 1.253285342E-002 9.833900980E-003 1.488715924E+001 7.009523771E-003 5.047284250E-003 4.175114261E-003 1.498158099E+001 9.130039857E-003 7.821733086E-003 5.468520082E-003 1.507600710E+001 7.655477471E-003 8.419114227E-003 6.068167882E-003 1.517043756E+001 1.262881086E-002 1.106001059E-002 8.743333551E-003 1.526487238E+001 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