# TOP STRAND, S1 # Oligo ID: R1441 # Sequence (5'to 3'): TGGCGAGCAC # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R1442 # Sequence (5'to 3'): GTGCTCGCCA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: Z9 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.020 # [KCl] = 0.0 # [Tris] = 0.002 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 05-21-2004 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- 1.687093083E+001 -6.589340000E-004 1.091759503E-002 7.632874500E-003 1.696544406E+001 2.156642500E-004 1.071292286E-002 8.281727500E-003 1.705996165E+001 -8.162666875E-004 8.956936616E-003 5.961336062E-003 1.715448360E+001 -2.916313037E-003 9.874971189E-003 6.772615463E-003 1.724900990E+001 1.397348200E-003 8.045182248E-003 6.293717050E-003 1.734354056E+001 -2.612258338E-003 7.843206058E-003 5.862263912E-003 1.743807557E+001 2.840276988E-003 9.151777392E-003 7.087152513E-003 1.753261494E+001 4.737920100E-003 9.415252686E-003 7.019760650E-003 1.762715867E+001 -1.800001625E-003 1.151135151E-002 6.810868312E-003 1.772170676E+001 1.414916475E-003 1.058935498E-002 7.177235275E-003 1.781625920E+001 -1.438331000E-003 9.771563276E-003 6.479125250E-003 1.791081599E+001 -6.038458500E-004 8.121269659E-003 4.835603962E-003 1.800537715E+001 -3.102864800E-003 1.074194736E-002 6.652682650E-003 1.809994266E+001 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