# TOP STRAND, S1 # Oligo ID: R1449 # Sequence (5'to 3'): CCAACTTCTT # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R1450 # Sequence (5'to 3'): AAGAAGTTGG # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: Q9 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.0015 # [KCl] = 0.0 # [Tris] = 0.002 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 03-24-2004 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- .1830000000E+02 .1498804086E-02 .6843306456E-02 .5529052212E-02 .1840000000E+02 -.1397312800E-02 .6848858769E-02 .5321542743E-02 .1850000000E+02 .5935280714E-03 .6604711322E-02 .4853989633E-02 .1860000000E+02 -.7242081429E-03 .9556134836E-02 .8355431306E-02 .1870000000E+02 .4601681429E-03 .8118477985E-02 .6687111592E-02 .1880000000E+02 -.1656887886E-02 .9326205816E-02 .7120912637E-02 .1890000000E+02 -.5048227143E-03 .1082598104E-01 .9005666327E-02 .1900000000E+02 -.2159177143E-03 .6513427929E-02 .5351946245E-02 .1910000000E+02 -.1182757571E-02 .5483212825E-02 .4682452367E-02 .1920000000E+02 -.3941952129E-02 .7380157461E-02 .5653578784E-02 .1930000000E+02 -.3140483891E-02 .9766536940E-02 .7115373807E-02 .1940000000E+02 -.4978411286E-02 .7566595161E-02 .5656278898E-02 .1950000000E+02 -.3794645214E-02 .8919834565E-02 .6917697020E-02 .1960000000E+02 -.4088352143E-02 .6802468415E-02 .6058592449E-02 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