# TOP STRAND, S1 # Oligo ID: R1457 # Sequence (5'to 3'): AGCGTAAGTC # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R1458 # Sequence (5'to 3'): GACTTACGCT # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: D1 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.125 # [KCl] = 0.0 # [Tris] = 0.010 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 02-10-2004 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- .1530000000E+02 .1679185644E-01 .1370334454E-01 .9689727852E-02 .1540000000E+02 .1917824566E-01 .1041304081E-01 .7363131770E-02 .1550000000E+02 .1136049935E-01 .5786011256E-02 .4091327795E-02 .1560000000E+02 .1520085302E-01 .8876480805E-02 .6276619770E-02 .1570000000E+02 .1297502916E-01 .6989822003E-02 .4942550537E-02 .1580000000E+02 .1827381210E-01 .1461413148E-01 .1033375147E-01 .1590000000E+02 .9773726192E-02 .1599000899E-02 .1130664379E-02 .1600000000E+02 .1222866360E-01 .3116872909E-02 .2203961970E-02 .1610000000E+02 .1317904776E-01 .6882673147E-02 .4866784855E-02 .1620000000E+02 .9755760078E-02 .4987909106E-02 .3526984353E-02 .1630000000E+02 .9058234524E-02 .7217762609E-02 .5103728886E-02 .1640000000E+02 .1237856257E-01 .3855600940E-02 .2726321570E-02 .1650000000E+02 .2036733585E-01 .1435365633E-01 .1014956772E-01 .1660000000E+02 .8208427949E-02 .3691832947E-02 .2610520112E-02 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