# TOP STRAND, S1 # Oligo ID: R1457 # Sequence (5'to 3'): AGCGTAAGTC # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R1458 # Sequence (5'to 3'): GACTTACGCT # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: Q9 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.0015 # [KCl] = 0.0 # [Tris] = 0.002 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 03-30-2004 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- 1.233941870E+001 -1.827002225E-002 1.656373278E-002 1.367742275E-002 1.243372283E+001 -1.258337350E-002 1.396075661E-002 1.034490975E-002 1.252803131E+001 -1.565735575E-002 1.466405842E-002 1.076051638E-002 1.262234416E+001 -2.143405725E-002 1.626272559E-002 1.190800913E-002 1.271666135E+001 -1.407740419E-002 1.548290958E-002 1.291366582E-002 1.281098291E+001 -1.232055200E-002 5.660027138E-003 3.945473000E-003 1.290530882E+001 -1.242776475E-002 9.553604857E-003 8.217680250E-003 1.299963908E+001 -8.102109250E-003 1.493538568E-002 1.209575575E-002 1.309397370E+001 -9.654712175E-003 1.367468703E-002 1.144374783E-002 1.318831268E+001 -1.203732725E-002 1.394934058E-002 1.137338775E-002 1.328265602E+001 -9.276244750E-003 8.025751161E-003 5.492990250E-003 1.337700371E+001 -1.352545580E-002 1.131685015E-002 9.393284200E-003 1.347135576E+001 -1.102867950E-002 1.143864239E-002 9.741540500E-003 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