# TOP STRAND, S1 # Oligo ID: R1457 # Sequence (5'to 3'): AGCGTAAGTC # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R1458 # Sequence (5'to 3'): GACTTACGCT # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: R9 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.010 # [KCl] = 0.0 # [Tris] = 0.002 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 05-04-2004 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- 9.889040458E+000 1.075299700E-002 7.552632470E-003 5.210088000E-003 9.983231320E+000 1.028209450E-002 5.466669254E-003 3.376043250E-003 1.007742654E+001 5.018509250E-003 3.459299529E-003 2.988349750E-003 1.017162611E+001 6.687957000E-003 1.042105123E-002 7.371481500E-003 1.026583005E+001 7.481335000E-003 1.239324533E-002 8.418512500E-003 1.036003833E+001 1.191057950E-002 9.948614060E-003 7.287665250E-003 1.045425098E+001 9.218403500E-003 4.343656148E-003 3.530936500E-003 1.054846798E+001 1.059158725E-002 6.360669246E-003 4.888772750E-003 1.064268934E+001 1.007972925E-002 5.236825393E-003 3.799786125E-003 1.073691505E+001 1.338547700E-002 3.808352533E-003 2.494346500E-003 1.083114512E+001 1.060487525E-002 5.580677220E-003 3.988197125E-003 1.092537955E+001 5.285709820E-003 4.564469190E-003 3.663115680E-003 1.101961833E+001 6.432955500E-003 1.134901000E-002 9.426029500E-003 1.111386147E+001 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