# TOP STRAND, S1 # Oligo ID: R1457 # Sequence (5'to 3'): AGCGTAAGTC # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R1458 # Sequence (5'to 3'): GACTTACGCT # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: X9 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.0005 # [KCl] = 0.0 # [Tris] = 0.002 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 03-16-2004 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- 1.158514251E+001 4.081937225E-003 3.148186583E-003 2.377748275E-003 1.167941178E+001 4.704932850E-003 5.143861427E-003 4.380304650E-003 1.177368542E+001 3.870684500E-003 7.970989314E-003 6.617468000E-003 1.186796341E+001 1.609436500E-003 9.013859224E-003 7.192745000E-003 1.196224575E+001 4.518990000E-004 7.893722094E-003 6.772545500E-003 1.205653246E+001 -1.661671500E-003 9.248695206E-003 7.585222500E-003 1.215082352E+001 3.568514500E-003 8.065847495E-003 6.514666000E-003 1.224511893E+001 2.348123500E-003 1.005918624E-002 7.434773250E-003 1.233941870E+001 -6.129901000E-003 1.153583646E-002 8.778782000E-003 1.243372283E+001 7.639274000E-003 1.143136348E-002 8.319994000E-003 1.252803131E+001 4.321941750E-003 1.105040499E-002 7.869845875E-003 1.262234416E+001 3.332235750E-003 7.849292369E-003 6.474451250E-003 1.271666135E+001 -3.756212875E-003 1.420721263E-002 9.405338563E-003 1.281098291E+001 1.541921500E-003 1.233553185E-002 7.995595750E-003 1.290530882E+001 2.560025433E-003 7.425845028E-003 5.820827568E-003 1.299963908E+001 -1.277090250E-003 1.028055044E-002 6.661105125E-003 1.309397370E+001 -6.062698750E-003 4.464947102E-003 3.332430625E-003 1.318831268E+001 -8.086809750E-003 2.890662926E-003 2.458936250E-003 1.328265602E+001 -8.994905750E-003 5.483335434E-003 4.727289250E-003 1.337700371E+001 -4.843525500E-003 7.302558327E-003 5.413808250E-003 1.347135576E+001 -5.203218680E-003 9.249752975E-003 6.791986820E-003 1.356571216E+001 -1.267220287E-002 9.358399362E-003 6.601820688E-003 1.366007292E+001 5.930005000E-004 8.582901384E-003 6.369899000E-003 1.375443804E+001 5.896265500E-003 1.539883199E-002 1.215790950E-002 1.384880751E+001 -3.483578000E-003 1.323674306E-002 1.132334700E-002 1.394318134E+001 7.919350000E-004 1.053786881E-002 7.719517500E-003 1.403755953E+001 -1.786630750E-003 8.238225474E-003 5.764627375E-003 1.413194207E+001 -7.132577250E-003 7.229088714E-003 5.870487750E-003 1.422632897E+001 8.525950000E-005 5.953544580E-003 5.108332500E-003 1.432072023E+001 -5.772004925E-004 3.974921023E-003 2.855944754E-003 1.441511584E+001 -9.604227500E-004 4.047635471E-003 2.937842875E-003 1.450951581E+001 2.368442500E-004 8.055978708E-003 6.603968750E-003 1.460392013E+001 -5.381276250E-003 4.946577478E-003 3.542459750E-003 1.469832881E+001 -5.848440750E-003 7.989948008E-003 6.106519250E-003 1.479274185E+001 -3.291174750E-003 1.787947391E-002 1.426106425E-002 1.488715924E+001 -2.501694500E-003 4.292237327E-003 3.634677500E-003 1.498158099E+001 2.279392375E-003 6.105227905E-003 4.442813813E-003 1.507600710E+001 2.074057750E-003 1.107259644E-002 8.425724750E-003 1.517043756E+001 3.353826500E-003 9.830298998E-003 7.765387000E-003 1.526487238E+001 -8.114880000E-004 6.641453487E-003 4.866916000E-003 1.535931156E+001 -3.351630500E-004 7.228490202E-003 5.179051525E-003 1.545375509E+001 -1.234064845E-002 1.042714833E-002 8.300046550E-003 1.554820298E+001 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