# TOP STRAND, S1 # Oligo ID: R1457 # Sequence (5'to 3'): AGCGTAAGTC # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R1458 # Sequence (5'to 3'): GACTTACGCT # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: Y9 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.003 # [KCl] = 0.0 # [Tris] = 0.002 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 04-10-2004 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- 1.215082352E+001 4.279892062E-003 3.764980161E-002 1.953670048E-002 1.224511893E+001 7.022800019E-003 2.791711004E-002 1.744705624E-002 1.233941870E+001 4.891930688E-003 3.627408022E-002 1.873477666E-002 1.243372283E+001 6.774890625E-003 4.065865372E-002 2.017998903E-002 1.252803131E+001 5.684591438E-003 3.138963739E-002 1.598651166E-002 1.262234416E+001 7.544430437E-003 3.530525413E-002 1.964929609E-002 1.271666135E+001 4.530036688E-003 3.185212559E-002 1.766843107E-002 1.281098291E+001 2.070077438E-003 2.888345357E-002 1.546329335E-002 1.290530882E+001 -4.805978125E-005 2.631116512E-002 1.464303583E-002 1.299963908E+001 4.311841625E-003 1.974855276E-002 1.287821516E-002 1.309397370E+001 4.313493631E-003 2.658867435E-002 1.355789251E-002 1.318831268E+001 1.495693606E-003 2.093534620E-002 1.210431392E-002 1.328265602E+001 -2.277655319E-003 1.459998801E-002 8.884047641E-003 1.337700371E+001 1.823518088E-003 2.086950445E-002 1.289018956E-002 1.347135576E+001 -7.496361875E-004 2.053101542E-002 1.351991449E-002 1.356571216E+001 2.510936938E-004 2.158452647E-002 1.374349207E-002 1.366007292E+001 -5.977254625E-003 1.389741083E-002 9.857088094E-003 1.375443804E+001 -2.629596925E-003 1.027793495E-002 7.394314344E-003 1.384880751E+001 4.769704094E-003 1.770553205E-002 1.008212268E-002 1.394318134E+001 7.170906875E-004 1.822353891E-002 1.218010316E-002 1.403755953E+001 8.717211938E-003 1.546996735E-002 1.101476518E-002 1.413194207E+001 3.733132563E-003 1.151177356E-002 9.143492258E-003 1.422632897E+001 6.154418806E-003 1.476146428E-002 9.937441020E-003 1.432072023E+001 5.843883169E-003 2.009684394E-002 1.205219004E-002 1.441511584E+001 5.080787500E-003 1.651896247E-002 8.902656813E-003 1.450951581E+001 1.023692869E-002 1.999134585E-002 1.370612131E-002 1.460392013E+001 6.340444025E-003 1.322257018E-002 1.001480998E-002 1.469832881E+001 4.895561331E-003 1.590082942E-002 9.548558585E-003 1.479274185E+001 1.323826086E-002 1.914418548E-002 1.238704821E-002 1.488715924E+001 9.357014738E-003 1.508352039E-002 1.075070810E-002 1.498158099E+001 3.945405706E-003 1.242446408E-002 8.926523220E-003 1.507600710E+001 2.443564266E-003 6.780803715E-003 4.551037075E-003 1.517043756E+001 6.616200756E-003 9.416504978E-003 7.424368058E-003 1.526487238E+001 8.690030688E-003 1.678704631E-002 1.081793974E-002 1.535931156E+001 7.187411875E-003 8.876744539E-003 6.838266328E-003 1.545375509E+001 6.310436250E-004 1.247280683E-002 9.371395672E-003 1.554820298E+001 2.585435312E-004 1.045851907E-002 8.170988535E-003 1.564265522E+001 9.861095125E-003 1.591858262E-002 1.031300266E-002 1.573711182E+001 3.536696979E-003 8.844659159E-003 7.232231769E-003 1.583157278E+001 2.977599856E-003 6.309491001E-003 4.823091662E-003 1.592603809E+001 2.513153106E-003 1.281502501E-002 7.089758295E-003 1.602050776E+001 9.237899575E-003 1.068757321E-002 7.002070444E-003 1.611498179E+001 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