# TOP STRAND, S1 # Oligo ID: R1457 # Sequence (5'to 3'): AGCGTAAGTC # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R1458 # Sequence (5'to 3'): GACTTACGCT # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: Z9 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.020 # [KCl] = 0.0 # [Tris] = 0.002 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 05-18-2004 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- 1.630394291E+001 1.663829000E-003 9.436100604E-003 8.043822500E-003 1.639843000E+001 -2.806341165E-003 4.533403356E-003 3.763328835E-003 1.649292146E+001 -4.256680750E-004 3.291524705E-003 2.427867425E-003 1.658741726E+001 -4.254657750E-004 5.780431870E-003 4.214015613E-003 1.668191743E+001 7.915225000E-005 3.247951919E-003 2.784002250E-003 1.677642195E+001 -2.924411250E-003 4.780330592E-003 3.273169250E-003 1.687093083E+001 1.871996675E-003 1.391534119E-003 1.031270325E-003 1.696544406E+001 -1.205271775E-003 4.461552059E-003 3.303026725E-003 1.705996165E+001 -1.040357250E-003 4.253190116E-003 3.650083750E-003 1.715448360E+001 -3.302377500E-004 1.863944158E-003 1.412976150E-003 1.724900990E+001 1.690412750E-004 3.111946423E-003 2.159516638E-003 1.734354056E+001 -6.509340175E-003 3.968299939E-003 2.804508738E-003 1.743807557E+001 -2.062535975E-003 4.315037569E-003 3.005788013E-003 1.753261494E+001 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