Welcome to IDT Biophysics

Owczarzy R, Moreira BG, You Y, Behlke MA, Walder JA (2008) Predicting stability of DNA duplexes in solutions containing magnesium and monovalent cations. Biochemistry, 47: 5336-5353.

This is a copy of the table from the publication. When you click on the specific melting temperature, you will obtain average melting profile (fraction of melted bases pairs versus temperature).

Table S1. Experimental melting temperatures of 74 DNA duplex oligomers in Tris-HCl buffers of various magnesium concentrations.
DNA sequence (5′ to 3′) Tm (oC) at 1M Na+ Tm (oC) at [Mg2+] indicateda
0.5mMb 1.5mMb 3.0mMb 10mMb 20mMb 50mMc 125mMc
aCt = 2 ± 0.2 µM. Buffers do not contain any KCl. b2mM Tris-HCl buffers.c10mM Tris-HCl buffers.
10-mers
ATCAATCATA 33.6 22.0 25.6 27.4 29.8 30.8 33.1 33.2
GAAATGAAAG 34.4 24.5 27.4 30.5 31.4 33.8 34.6 35.3
CCAACTTCTT 40.6 30.1 35.3 36.5 39.3 39.8 40.4 40.3
AGCGTAAGTC 40.3 29.7 36.2 37.6 39.3 40.8 42.2 42.4
CGATCTGCGA 49.1 38.4 43.2 44.0 47.6 47.6 47.3 47.6
TGGCGAGCAC 55.3 45.2 49.7 50.6 52.8 53.3 54.2 54.4
15-mers
TACTAACATTAACTA 51.1 39.3 42.9 44.4 47.1 47.4 48.3 49.0
ATACTTACTGATTAG 51.5 39.9 43.6 45.0 46.8 47.9 48.6 49.2
GTACACTGTCTTATA 54.8 43.4 47.2 48.6 50.6 51.2 52.1 52.5
GTATGAGAGACTTTA 55.4 43.5 47.0 48.6 51.0 51.5 52.3 52.8
AGTAGTAATCACACC 57.1 46.5 49.5 51.0 53.0 53.4 54.0 54.5
ATCGTCTCGGTATAA 58.6 48.0 51.0 52.7 54.8 55.1 55.6 56.3
ACGACAGGTTTACCA 61.3 51.6 54.5 56.0 58.1 58.6 59.0 59.5
CTTTCATGTCCGCAT 62.8 52.7 55.2 56.5 58.4 59.0 59.5 59.6
TGGATGTGTGAACAC 60.4 49.9 53.4 54.8 56.6 57.3 57.9 58.3
ACCCCGCAATACATG 62.9 52.7 55.3 56.6 58.5 59.0 58.9 59.2
GGTCCTTACTTGGTG 60.3 50.4 53.3 54.8 56.6 57.2 57.4 57.5
CGCCTCATGCTCATC 65.8 54.3 57.2 58.7 60.1 60.6 61.3 61.1
AAATAGCCGGGCCGC 70.4 60.4 63.4 64.6 66.1 66.4 67.0 67.1
CCAGCCAGTCTCTCC 66.7 56.6 59.4 60.5 62.1 62.5 62.9 63.0
GACGACAAGACCGCG 68.6 59.4 61.7 62.8 64.1 64.6 65.3 64.9
CTCGCGGTCGAAGCG 70.7 61.3 63.9 65.1 66.3 66.9 67.3 66.8
GCGTCGGTCCGGGCT 74.1 65.2 67.5 68.5 69.5 70.3 70.6 70.2
20-mers
TATGTATATTTTGTAATCAG 59.8 48.6 51.4 52.6 54.4 55.1 55.8 56.3
TTCAAGTTAAACATTCTATC 61.5 49.9 52.6 53.9 55.6 56.4 57.2 57.8
GAGATTGTTTCCCTTTCAAA 65.3 53.2 56.2 57.5 59.2 60.1 60.9 61.3
ATGCAATGCTACATATTCGC 68.9 57.4 59.9 61.0 62.5 63.1 63.8 63.7
CCACTATACCATCTATGTAC 64.4 52.9 55.4 56.6 58.0 58.7 59.2 59.5
CCATCATTGTGTCTACCTCA 68.5 58.1 60.4 61.5 62.9 63.5 64.0 64.1
CGGGACCAACTAAAGGAAAT 68.5 57.6 60.1 61.5 62.9 63.4 64.0 64.4
TAGTGGCGATTAGATTCTGC 71.2 60.0 62.5 63.6 64.9 65.6 66.2 66.5
TACTTCCAGTGCTCAGCGTA 73.6 62.2 64.7 65.8 67.3 67.7 68.3 68.4
CAGTGAGACAGCAATGGTCG 72.5 61.4 63.9 65.0 66.2 66.8 67.2 67.0
CGAGCTTATCCCTATCCCTC 70.3 60.0 62.5 63.6 65.0 65.5 65.9 65.9
CGTACTAGCGTTGGTCATGG 71.1 61.1 63.4 64.4 65.8 66.2 66.5 66.5
AAGGCGAGTCAGGCTCAGTG 76.3 66.2 68.5 69.8 70.6 71.2 71.6 71.4
ACCGACGACGCTGATCCGAT 77.3 66.2 68.7 69.5 71.0 71.0 71.6 71.2
AGCAGTCCGCCACACCCTGA 78.5 67.6 69.9 70.6 72.1 72.5 72.7 72.4
GTGGTGGGCCGTGCGCTCTG 81.0 69.8 72.0 73.2 74.4 74.6 75.0 74.4
GTCCACGCCCGGTGCGACGG 81.1 70.3 72.5 73.9 74.0 74.5 74.7 74.3
25-mers
GATATAGCAAAATTCTAAGTTAATA 66.1 54.2 56.8 58.0 59.7 60.9 61.2 61.8
ATAACTTTACGTGTGTGACCTATTA 71.8 59.9 62.4 63.4 64.9 65.4 66.1 66.4
CCCTGCACTTTAACTGAATTGTTTA 72.5 60.7 63.2 64.2 65.6 66.1 66.8 67.1
TAACCATACTGAATACCTTTTGACG 71.3 59.7 62.1 63.1 64.5 65.1 65.7 65.8
TCCACACGGTAGTAAAATTAGGCTT 73.8 62.4 64.7 65.7 67.0 68.1 68.1 68.2
TTCCAAAAGGAGTTATGAGTTGCGA 73.8 62.1 64.4 65.5 66.9 67.4 67.9 68.1
AATATCTCTCATGCGCCAAGCTACA 76.5 64.5 67.0 67.8 69.2 69.5 70.3 70.1
TAGTATATCGCAGCATCATACAGGC 75.0 62.9 65.1 66.1 67.9 67.9 68.4 68.3
TGGATTCTACTCAACCTTAGTCTGG 73.6 62.3 64.5 65.6 66.9 67.3 67.9 67.8
CGGAATCCATGTTACTTCGGCTATC 74.8 64.0 66.2 67.2 68.5 68.9 69.5 69.5
ACAGCGAATGGACCTACGTGGCCTT 81.0 69.9 71.9 72.9 73.9 74.2 74.4 74.3
AGCAAGTCGAGCAGGGCCTACGTTT 81.5 70.3 72.3 73.3 74.2 74.8 75.0 75.0
GCGAGCGACAGGTTACTTGGCTGAT 80.1 69.4 71.5 72.4 73.4 73.9 74.2 74.0
AAAGGTGTCGCGGAGAGTCGTGCTG 82.4 70.9 73.1 73.9 75.2 75.6 75.7 75.5
ATGGGTGGGAGCCTCGGTAGCAGCC 83.4 72.2 74.5 75.3 76.4 76.7 76.8 76.7
GCCAACTCCGTCGCCGTTCGTGCGC 84.6 73.8 75.7 76.5 77.2 77.5 77.7 77.5
ACGGGTCCCCGCACCGCACCGCCAG 88.3 77.3 79.0 79.7 80.2 80.5 80.8 80.3
30-mers
TTATGTATTAAGTTATATAGTAGTAGTAGT 66.6 55.0 57.5 58.5 60.0 60.6 61.3 61.7
ATTGATATCCTTTTCTATTCATCTTTCATT 70.4 58.8 61.2 62.3 63.8 64.4 65.1 65.6
AAAGTACATCAACATAGAGAATTGCATTTC 73.2 61.5 63.8 64.7 65.9 66.5 67.2 67.5
CTCAACTTGCGGTAAATAAATCGCTTAATC 75.5 64.1 66.1 67.2 68.3 68.8 69.6 69.8
TATTGAGAACAAGTGTCCGATTAGCAGAAA 76.4 65.3 67.4 68.4 69.6 70.1 70.7 70.8
GTCATACGACTGAGTGCAACATTGTTCAAA 76.9 65.5 67.6 68.5 69.8 70.1 70.7 70.8
AACCTGCAACATGGAGTTTTTGTCTCATGC 78.7 67.9 69.9 70.8 71.7 72.3 72.8 72.9
CCGTGCGGTGTGTACGTTTTATTCATCATA 77.6 66.7 68.7 69.6 70.8 71.2 71.6 71.5
GTTCACGTCCGAAAGCTCGAAAAAGGATAC 78.7 68.1 70.2 71.2 72.2 72.7 73.2 73.4
TCGGAGAAATCACTGAGCTGCCTGAGAAGA 80.9 69.0 71.0 71.9 72.9 73.3 73.8 73.7
CTTCAACGGATCAGGTAGGACTGTGGTGGG 80.1 68.3 70.1 71.0 72.0 72.5 72.9 72.9
ACGCCCACAGGATTAGGCTGGCCCACATTG 84.0 73.2 75.0 75.8 76.7 76.9 77.4 77.2
GTTATTCCGCAGTCCGATGGCAGCAGGCTC 84.1 72.9 74.8 75.5 76.6 76.9 77.3 77.0
TCAGTAGGCGTGACGCAGAGCTGGCGATGG 84.6 73.7 75.8 76.0 77.0 77.7 78.0 77.7
CGCGCCACGTGTGATCTACAGCCGTTCGGC 84.5 73.9 75.3 76.0 77.1 77.0 77.4 77.0
GCCCCTCCACTGGCCGACGGCAGCAGGCTC 87.7 77.6 79.1 79.8 80.4 80.8 80.9 80.6
CGCCGCTGCCGACTGGAGGAGCGCGGGACG 88.6 78.7 80.1 80.9 81.1 81.6 81.6 81.2